Genetic Diagnosis of Entamoeba Histolytica in Patients with Acute Diarrhea in AL-Rifai City/Thi-Qar province

Abstract

Entamoeba histolytica is one of the Entamoeba histolytica is one of the most prevalent parasitic infections in humans, causing amoebiasis and liver abscess, most prevalent parasitic infections in humans, causing amoebiasis and liver abscess, with diarrhoea being one of the most common symptoms. Diarrhea is a symptom of a digestive disorder that can be caused by pathogens or be the result of an imbalance in the digestive process. Diarrhea is a condition in which you defecate three or more times a day and your stool is watery and liquid. In Thi-Qar Province / AlRifea city, this study focused on identifying and diagnosing Entamoeba histolytica from samples of patients with diarrhoea cases, a combination of microscopic and genomic methods. From October 2017 to January 2018, stool samples were collected from patients with infectious diarrhoea at Al-Rifai General Hospital for an extended length of time (603) stool samples were collected from patients of various ages and genders for the detection of Entamoeba histolytica. The percentage of positive samples was (27.9%), while the percentage of negative samples was determined by microscopy (72.1%). Males had (28.2 %) favourable outcomes, while females had (27.3 %) positive results. In terms of housing, rural areas had the highest infection rate (38.1 %). Considering the low infection prevalence in urban areas (27.9%). In terms of age, the lowest infection rate (21.1%) was discovered in the age group. (21–30) years, while the greatest infection rate (37.8%) was identified in the age group fewer than ten years. While the results by PCR examination show that percentage of positive samples are was (19), with percentage of (19.8) %, while negative samples were (77), with a percentage of (77.8%) Males had the highest infected patients. (20.3%), while females had the lowest (19.0%) Infected patients in rural areas were (38.1 %) and in urban areas were (27.9 %), respectively. In terms of age, infected patients were discovered (31.2 %) in the age group under 10 years and (6.2 %) in the age group between (11 - 20) years.