Abstract
This study aim is to determine the incidence and the virulence of Aeromonas hydrophila in raw milk, randomly collected from Basra governorate by using of polymerase chain reaction (PCR) technique. In this study, the total number of raw milk samples collected from cows, buffaloes and goats that kept from different the regions of Basra governorate were 90 samples. The PCR technique is modern method which regarded as a reliable tool to detect virulent gene of the A. hydrophila isolates. The PCR assays using the primers sets SerAh-F and SerAh-R resulted in the amplification of 650-bp bands from the targeted proteases gene of the A. hydrophil. The result of the present study showed that the results of PCR concerning the proteolytic activity of A. hydrophila in the tested raw milk samples according to animals' source. The higher percentage of the proteolytic activity was found in the cow's raw milk samples 40% and in the buffalo's milk samples was 26.7% while, the proteolytic activity did not find in the goat's milk samples. The association between the source of the milk sample and proteolytic A. hydrophila positive results was considered to be statistically highly significant. The higher percentage of the A. hydrophila isolates found in the raw cow milk was 40%, and the A. hydrophila isolates found in the raw buffalo milk was 26.7%, while, the A. hydrophila isolates did not find in the goat milk.
Highlights
Aeromonas species are facultative anaerobic gramnegative microscopic organisms
Aeromonas has the ability to produce the various virulent factors which included the different types of hemolysins such asaerolysin, proteases, adhesions, invasins, a heat labile enterotoxin, phospholipase, and lipase [4,5]
Lots of proteases delivered by psychrotrophic microscopic organisms are steady even under ultra-high temperature (UHT) condition and proceed actively in dairy products [6]
Summary
Aeromonas species are facultative anaerobic gramnegative microscopic organisms. It related to the family Aeromonadaceae, which is frequently isolated from various food and environmental sources including sea, river, fresh and ground water [1]. Lots of proteases delivered by psychrotrophic microscopic organisms are steady even under ultra-high temperature (UHT) condition and proceed actively in dairy products [6]. Its developing worry as a blood borne pathogen A. hydrophila could take an interest in disintegration of the food store at refrigeration temperature due to its capacity to grow up and make thermo-resistant extracellular proteins (lipase, protease, amylase, and nuclease) that is ready to defiling significant milk constituent and change the nature of completed dairy products [12]. Any way little is perceived concerning the role of milk in the spread of these pathogens so this investigation was embrace as a component of an endeavor to decide the role of milk in the study of disease transmission of Aeromonas contamination, the propriety of the specific media for the restoration of the living being, the proteolytic action of detached A. hydophilia
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