Genetic control of glycolipid expression

Abstract

A polymorphic variation of sialic acid species of sialosyllactosylceramide was found in dog erythrocytes. The analysis of the glycolipids in the erythrocytes of the individual dogs in a family of a Japanese breed of dog, Shiba-Inu, showed that the expression of sialosyllactosylceramide containing N-glycolylneuraminic acid was an autosomal dominant trait over the expression of that containing N-acetylneuraminic acid. Polymorphic variations of major liver gangliosides were also found in various strains of inbred mice. The strains were classified into three groups; the first group possessed only II 3 Neu-Gc-LacCer, the second group possessed II 3NeuGc-GgOse 3Cer in addition to II 3NeuGc-LacCer and the third group possessed II 3NeuGc-GgOse 4Cer and II 3NeuGc,IV 3NeuGc-GgOse 4Cer as well as the above two gangliosides. By subjecting mice of these three groups to genetic analysis, the strain of the first group (WHT/Ht mice) was demonstrated to be a recessive homozygote which had a single autosomal defective gene making it unable to express N-acetylgalactosaminyltransferase activity to produce II 3NeuGc-GgOse 3Cer. The strains of the second group (BALB/c and C57BL/10 mice) were also demonstrated to be recessive homozygotes which had a single autosomal defective gene making them unable to express high enough level of galactosyltransferase activity to produce II 3NeuGc-GgOse 4Cer. By the analysis of gangliosides and the enzyme activity of H-2 congenic mice and mice produced by a mating, this defective gene controlling the expression of II 3NeuGc-GgOse 4Cer through the regulation of the transferase activity was demonstrated to be linked to H-2 complex on chromosome 17.