Abstract

Analysis of glucose phosphate isomerase (GPI) zymograms extracted from grains of wheat aneuploids indicated that the three Gpi-1 loci each control the production of at least two enzyme subunits. Flat bed isoelectric focussing revealed the 21 possible GPI dimers as 11 discrete bands. Although no varietal differences were detected in the Gpi-1 products, a null-allele at the Gpi-D1 locus was found in some stocks of Chinese Spring. Analysis of crosses segregating for this Gpi-D1b phenotype indicated that the genes encoding the enzyme subunits are closely linked and comprise a compound locus. GPI zymograms were used to identify homoeologous loci in chromosome additions of rye and Hordeum chilense to wheat, and to confirm the genotypes of chromosome substitutions of Aegilops umbellulata in wheat.

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