Abstract
Genetic characterization of strains of Streptococcus iniae recovered from morbidity and mortality of farmed rainbow trout in different provinces of Iran were studied. The Gram-positive cocci isolates were obtained from the kidney tissues of diseased rainbow trout on blood agar at 25°C for 72 h. The grown bacteria were then characterized using biochemical and molecular works. The identified 26 isolates of S. iniae producing a 513 bp in PCR procedure were then compared using random amplified polymorphic DNA (RAPD) analysis using 9 random primers. The phylogenetic tree of the RAPD product using UPMGA software included these strains in one genetic group but into two clusters. The results of this study show that S. iniae strains from the diseased rainbow trout in the north part of Iran are genetically similar to those strains in the south and west parts of the country.
Highlights
Streptococcus iniae is one of the major causative agents of streptococcosis in aquaculture industry and is an important zoonotic bacterial disease causing morbidity and mortality in humans [1,2,3,4,5]
Adequate studies have focused on the immunepathogenesis of the infection, minimal data is available on the genetic characterization on genetic diversity of the isolated strains of this bacterium in fish [3, 9]
These 26 isolates were subjected to PCR for further confirmation and the obtained results showed that all isolates were S. iniae giving a band of 513 bp for PCR products (Figure 1)
Summary
Streptococcus iniae is one of the major causative agents of streptococcosis in aquaculture industry and is an important zoonotic bacterial disease causing morbidity and mortality in humans [1,2,3,4,5]. Adequate studies have focused on the immunepathogenesis of the infection, minimal data is available on the genetic characterization on genetic diversity of the isolated strains of this bacterium in fish [3, 9]. The importance of this is to provide an effective method of mass vaccination covering a number of isotypes and vaccination is one of the most feasible ways to prevent the losses due to this zoonotic bacterial disease in aquaculture industry [10]. The aim of this study was to compare the recovered isolates of S. iniae at molecular level to determine if intraspecific variants could be found among the isolates from different geographical locations of Iran which is a big land with different climates and environmental conditions
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