Genetic characterization of extensive drug resistant Acinetobacter baumannii: an appalling impediment.

Faeze Abbaszadeh,Mohammad Ahangarzadeh Rezaee,Javid Sadeghi,Alka Hasani,Abolfazl Vahhabi,Akbar Hasani,Mahin Ahangar Oskouee

doi:10.3897/folmed.63.e56566

Faeze Abbaszadeh, Mohammad Ahangarzadeh Rezaee + Show 5 more

Open Access

https://doi.org/10.3897/folmed.63.e56566

Copy DOI

Journal: Folia medica	Publication Date: Oct 31, 2021
License type: CC BY 4.0

Affiliation: Tabriz University of Medical Sciences

Abstract

Acinetobacter baumannii infections are a growing public-health concern. The bacterium's potentiality to acquire resistance to a number of commonly used antibiotics has turned it into a formidable pathogen. Molecular characterization of extensive drug resistant (XDR) typing of A. baumannii clinical isolates by polymerase chain reaction. Thirty XDR A. baumannii were investigated for the presence of genes encoding carbapenemase resistance, biofilm capacity, autoinducer synthase, virulence and surface motility by polymerase chain reaction (PCR). Later, the isolates were typed by plasmid-based replicon (Rep) (PBRT) and trilocus sequence typing. All 30 XDR A. baumannii strains displayed genes related to surface motility, autoinducer synthase, virulence determinant, biofilm related genes except PER, and bap, the frequency of which was 83.3% and 76.6%, respectively. Analysis of rep genes showed highest frequency of rep6 and rep2 genes, with frequency of 75% and 65%, respectively. All XDR A. baumannii strains belonged to SG I (European clone II) group. Our results show the extraordinary plasticity of XDR A. baumannii and suggest that the strains have gained endemicity in our hospital, which could be a great concern in the near future.

Highlights

Acinetobacter baumannii infections are a growing public-health concern
Of 30 XDR A. baumannii clinical isolates enrolled in the study, 16 (53.3%) isolates were obtained from male patients and 14 (46.6%) from females
Since in our research study, all A. baumannii strains were positive for at least one of the biofilm-associated genes abaI may be positive in all strains

Summary

Introduction

Acinetobacter baumannii infections are a growing public-health concern. The bacterium’s potentiality to acquire resistance to a number of commonly used antibiotics has turned it into a formidable pathogen. The organism possesses intrinsic and acquired resistance to a number of commonly used antibiotics and is gradually bequeathing intra and inter-hospital setting.[2] antibiotic resistance rates can vary according to the country, the individual hospital, and may even depend on biological, epidemiological or methodical factors.[3] As the organism has gained hold of the antibiotic resistance, it is not uncommon to find multidrug-resistant (MDR, resistance to at least three classes of antimicrobials), extensively drug-resistant (XDR, MDR plus resistance to carbapenems), and pan-drug-resistant (PDR, XDR plus resistance to polymyxins) nosocomial isolates that are hard to treat with the currently available drugs.[4] Amongst all these, according to World Health Organization (WHO) carbapenem-resistant A. baumannii (CRAB) strains have emerged as one of the most concerning antibiotic-resistant pathogens among other gram-negative bacteria.[5]. Strains carrying blaOXA-58 stand out as the most common type from Europe, Argentina, Australia, the United States and many Asian countries.[7]

Objectives

Results

Discussion

Conclusion