Abstract
Aim: To characterize one of the major glycoprotein genes viz., glycoprotein C (gC; UL44, unique long region 44) of bovine herpesvirus 1(BoHV1) of Indian origin at genetic and phylogenetic level. Materials and Methods: A bovine herpesvirus 1 isolate viz., (BoHV1/IBR 216 II/ 1976/ India) maintained at Division of Virology, IVRI, Mukteswar was used for the current study. The DNA was extracted using commercial kit and the complete ORF of gC gene was amplified, cloned, and sequenced by conventional Sanger sequencing method. The sequence was genetically and phylogenetically analysed using various bioinformatic tools. The sequence was submitted in the Genbank with accession number Kc756965. Results: The complete ORF of gC gene was amplified and sequenced. It showed 100% sequence homology with reference cooper strain of BoHV1 and divergence varied from 0% to 2.7% with other isolates of BoHV1. The isolate under study had divergence of 9.2%, 13%, 26.6%, and 9.2% with BoHV5 (Bovine herpesvirus 5), CvHV1 (Cervid herpesvirus 1), CpHV1 (Caprine herpesvirus 1), and BuHV1 (Bubaline herpesvirus 1), respectively. Conclusion: This is the first genetic characterization of complete open reading frame (ORF) of glycoprotein C gene (UL44) of Indian isolate of BoHV1. The gC gene of BoHV1 is highly conserved among all BoHV1 isolates and it can be used as a target for designing diagnostic primers for the specific detection of BoHV1.
Highlights
Bovine herpesvirus 1 (BoHV1), the causative agent of infectious bovine rhinotracheitis/infectious pustular vulvovaginitis (IBR/IPV), infects mainly cattle and buffaloes, but sheep and goats, swine, yaks, mithuns, mink and ferrets are affected [1,2,3]
The glycoprotein C (gC) gene of BoHV1 is highly conserved among all BoHV1 isolates and it can be used as a target for designing diagnostic primers for the specific detection of BoHV1
The present study describes the complete sequences of gC genes of BoHV1 which was isolated in 1976 from calf suffering from keratoconjunctivitis [9]
Summary
Bovine herpesvirus 1 (BoHV1), the causative agent of infectious bovine rhinotracheitis/infectious pustular vulvovaginitis (IBR/IPV), infects mainly cattle (both domestic as well as wild) and buffaloes, but sheep and goats, swine, yaks, mithuns, mink and ferrets are affected [1,2,3]. BoHV1 causes mainly three clinical syndromes in affected cattle and buffalos viz. Infectious bovine rhinotracheitis (IBR), infectious pustular vulvovaginitis (IPV), and infectious pustular balanopostitis (IPB) [4]. The BoHV1 genome encodes 73 recognized open reading frames (ORFs) within a 135301 bp doublestranded DNA genome. Three subtype variants of BoHV1, designated BoHV1.1a, BoHV1.2a and BoHV1.2b, are recognized based on genomic DNA restriction endonuclease profiles and clinical observations [5].
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