Abstract
This paper reports the characterization of naturally occurring rhizobacteria from NE India for the control of wilt and damping-off diseases in French beans. French bean (Phaseolus vulgaris L.) is one of the most important food legumes for direct human consumption which was originated from South and Central America. In NE India wilt and damping-off in beans are serious diseases which are mainly caused by Fusarium spp and Rhizoctonia solani. Out of 625 bacterial isolates obtained from French bean growing fields of Manipur, 15 potential isolates were selected, whose inhibition zone width were more than 15 mm. Strain IBSD-LKBI exhibited strong antagonistic activity and can inhibit both the pathogens. Besides, this strain also produced extracellular hydrolytic enzymes (protease, chitinase, β-1, 3-glucanase), secondary metabolites (Siderophore, HCN). It also produces plant growth promoting hormone (IAA) and can solubilize organic phosphate. Burkholderia cepacia IBSD-LKBI exhibited the highest plant growth promotion and BCA. After 60 days of treatment, plant height, root length, fresh weight and % of disease suppression were significantly (P Burkholderia cepacia (98%). Multiple sequence alignment & phylogram with closely related sequence accessions using MEGA Software revealed this naturally occurring biocontrol bacteria belongs to Burkholderia cepacia complex genomovar I based on the Biolog identification system with 16S rDNA gene sequence analysis. This organism is one of the new reports from NE India for the control of wilt and damping-off diseases in French beans.
Highlights
One of the obstacles in the production of high quality and yield of food crops is the difficulty to control fungal diseases in plant
This paper reports the characterization of naturally occurring rhizobacteria from NE India for the control of wilt and damping-off diseases in French beans
Multiple sequence alignment & phylogram with closely related sequence accessions using MEGA Software revealed this naturally occurring biocontrol bacteria belongs to Burkholderia cepacia complex genomovar I based on the Biolog identification system with 16S rDNA gene sequence analysis
Summary
One of the obstacles in the production of high quality and yield of food crops is the difficulty to control fungal diseases in plant. In North East region of India the yield of French beans are severely affected due to fungal infection by Fusarium oxysporum and R. solani causing charcoal rot, wilt and damping off diseases. Fusarium and R. solani are the most common and destructive plant pathogens and a monotypic soil borne species with a wide host range involving more than 200 species in 75 families [2]. These fungi infect plants from seedling stage to maturity. Microsclerotia is a compact mass of hardened mycelium stored with reserve food material that becomes detached and remains dormant until a favourable opportunity for growth occurs
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