Abstract

Twelve microsatellite loci previously developed in the tropical tree Cariniana estrellensis were genetically characterized in Cariniana legalis. Polymorphisms were assessed in 28 C. legalis individuals found between the Pardo and Mogi-Guaçu River basins in the state of São Paulo, Brazil. Of the 12 loci, 10 were polymorphic and exhibited Mendelian inheritance. The allelic richness at each locus ranged from 2-11, with an average of 7 alleles per locus, and the expected heterozygosity ranged from 0.07-0.88. These loci showed a high probability of paternity exclusion. The characteristics of these heterologous microsatellite markers indicate that they are suitable tools for investigating questions concerning population genetics in C. legalis.

Highlights

  • Cariniana legalis (Mart.) Kuntze, commonly known as jequitibá rosa, is a woody tree species of the Lecythidaceae family with late successional characteristics (Lorenzi, 2002)

  • Allozymes have been surveyed in C. legalis for purposes such as assessing its mating system (Sebbenn et al, 2000), very little is known about the finescale population structure of this species

  • Guidugli et al (2008) developed 15 polymorphic microsatellite markers for Cariniana estrellensis and demonstrated the transferability of 12 of these primer pairs to C. legalis based on amplification of just seven individuals

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Summary

Introduction

Cariniana legalis (Mart.) Kuntze, commonly known as jequitibá rosa, is a woody tree species of the Lecythidaceae family with late successional characteristics (Lorenzi, 2002). Guidugli et al (2008) developed 15 polymorphic microsatellite markers for Cariniana estrellensis and demonstrated the transferability of 12 of these primer pairs to C. legalis based on amplification of just seven individuals. In this study, characteristics of 12 heterologous loci were investigated using 28 C. legalis individuals in order to confirm the general applicability of these markers for genetic studies of this species.

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