Genetic Characterization, Agro-Morphological and Physiological Evaluation of Grafted Tomato under Salinity Stress Conditions

Emad A Abdeldym,Mohamed A M Atia,Hoda R L Abdellateaf,Mohamed M El-Mogy

doi:10.3390/agronomy10121948

Emad A Abdeldym, Mohamed A M Atia + Show 2 more

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https://doi.org/10.3390/agronomy10121948

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Abstract

This study aims to determine grafting’s efficiency to improve tomato growth and production under salinity stress conditions. A commercial tomato hybrid (cv. Bark) and eight wild tomato accessions were evaluated at molecular, physiological and agronomic levels. At the molecular level, two robust gene-targeting marker systems (Conserved DNA-Derived Polymorphism; CDDP and Start Codon Targeted Polymorphism; SCoT) were employed. Bark cv. was grafted as a scion onto the four tomato genotypes’ roots as stocks. The rootstocks effect was evaluated by growing plants at 0, 100 and 200 mM NaCl. Our results showed that grafting enhanced plant shoots and roots growth (plant height, number of branches, plant fresh weight, root length, and root fresh and dry weight), fruit yield (total yield, number and weight of fruits) and fruit quality (Vitamin C, firmness and total soluble solids) in Bark on most tested rootstocks. A significant interaction between salinity levels and rootstocks for all measured hormones, antioxidants and proline was observed. In conclusion, our consistent results from the three approaches (molecular, physiological and agronomical) revealed that the four genotypes (LA1995, LA2711, LA2485 and LA3845) were found to be grouped and exhibit better performance under salinity stress conditions. Furthermore, grafting could be a low-cost alternative method to improve salt tolerance in sensitive tomato genotypes.

Highlights

Tomato (Solanum lycopersicum L.) is considered one of the most important vegetable crops grown worldwide due to its economic and health importance
Conserved DNA-Derived Polymorphism (CDDP)–2, 4 and 7), with percentages of polymorphism ranging from 16.7% to 35%
Genome-wide conserved regions across diverse plant species have sped the development of several functional markers such as CDDP and Start Codon Targeted Polymorphism (SCoT)

Summary

Introduction

Tomato (Solanum lycopersicum L.) is considered one of the most important vegetable crops grown worldwide due to its economic and health importance. According to a statement by the FAO in 2018. (http://faostat.fao.org/), the world production of tomato was 182,258,016 tons, which was harvested from. The salinity of soil and water became a severe universal problem restricting the growth, productivity and quality of most crops [2–4]. In the coming few decades, the percentage of affected land will increase due to a reduction in the quantity and quality of available irrigation water and global climate change [5]. Twenty percent of the global cultivated lands and 33% of the irrigated agricultural lands suffer from high salinity [6]

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