Genetic characterisation of the mithun (Bos frontalis) and studies of spermatogenesis, blood groups and haemoglobins of its hybrids with Bos indicus

Abstract

The mithun (Bos frontalis) and its hybrids with Bos indicus were studied to provide further cytogenetic information which might throw light on the mechanisms of the male hybrid infertility and facilitate the establishment of a stable crossbreed. It was shown that compared with conventional cattle the mithun has a homozygous, species specific 2/27 centric fusion which reduced the diploid chromosome number from 60 to 58. This provided further proof that Robertson translocation-type rearrangements have been the major source of interspecies karyotype differences in the evolution of the Bovidae. In the mithun there was also significant polymorphism between centromeres of non-homologous chromosomes and there was heteromorphism between several homologous chromosomes which could possibly serve as useful genetic markers for breeding programmes. In F1 hybrids spermatogenesis progressed to a relatively advanced stage, without going so far as to produce spermatozoa. In back crosses to B indicus spermatogenesis progressed further so that spermatozoa could be seen, though not as numerous as in normal bulls. In most hybrids there were haemoglobin bands which corresponded either to Hb A or Hb B of cattle but were much wider. It was shown that these were a combination of Hb Mi derived from the mithun and Hb A or Hb B derived from B indicus. In a few hybrids there were only Hb Mi. In these cases Hb Mi had been present in both parents and proved that the dam was not a pure siri. The possible mechanism of hybrid male infertility is discussed including faults in the epistatic gene effect between chromosomes and changes in the degree of association of centromeric regions in interspecies hybrids. It is suggested that additional cytogenetic examination of blood lymphocytes and especially of testicles would help the understanding of the fertility barriers of hybrid males and would make a breeding programme for a stable crossbreed possible.