Abstract
Diarrhoeal disease continues to be an important cause of morbidity and mortality among neonates of various animal species worldwide. Amongst several viral agents associated with diarrhoea, rotavirus (RV) has been recognised as the major aetiological agent. In the following study, 184 faecal samples from diarrhoeic bovine claves were collected from different dairy farms of Madhya Pradesh (M.P), central India. Of these, 25 samples (13.6%) were found to be positive for RV infection using RNA polyacrylamide gel electrophoresis and reverse-transcription-polymerase chain reaction. One of the isolates (cow/C-32/IND/2008/G10) of predominant genotype (G10) was selected for sequence and SimPlot analysis. The results suggested occurrence of group-A RV in bovine population of central India, with circulation of a G10 genotype, which showed sequence identity of 63-98% with other bovine, human and porcine G10 isolates from India and other parts of the world. The C-32 isolate showed maximum sequence identity (98%) with a RV strain isolated from water buffaloes of Punjab (BRV 73Acc. No. EU311194). Our isolate showed minimum sequence identity (6372%) with G10 bovine isolates from the eastern region of the country (RUBVM135, EF200544 and EF200543). The results confirm circulation of divergent strains of G10 genotypes in India and warrants further molecular studies to understand the evolution of divergent G10 virus population in nature.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.