Abstract
Investigations were carried out to detect a blocked step in the glycerol biosynthesis of the glycerol auxotroph GL-21 derived from Corynebacterium alkanolyticum No. 314.The enzyme required for the conversion of dihydroxyacetone phosphate to α-glycerophosphate was assumed to be defective, because some of glycerol derivatives and glycerol analogues substituted for glycerol as a growth factor, in which glycerides, phospholipids, surfactants and intermediary metabolites in the glycolysis pathway were included.To confirm this assumption, the activities of α-glycerophosphate dehydrogenase of the mutant were compared to those of the parent. From these results, the auxotroph GL-21 was found to be deficient for a specific l-glycerol-3-phosphate: NADP oxidoreductase which is indispensable for the synthesis of glycerol.
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