Genetic association of ABCC8 rs757110 polymorphism with Type 2 Diabetes Mellitus risk: A case-control study in South India and a meta-analysis

Abstract

Type 2 Diabetes Mellitus (T2DM) is a metabolic disorder characterized by impaired insulin secretion leading to various complications. The case-control study enrolled 210 T2DM and 200 non-diabetic subjects belonging to south India for determining the role of rs757110 single nucleotide polymorphism (SNP) of ABCC8 gene in T2DM using amplification refractory mutation system (ARMS)-PCR and DNA sequencing. The results of case-control study were used to conduct a meta-analysis based on published literature to elucidate the association of rs757110 polymorphism with T2DM by calculating their pooled odds ratios (ORs) and 95% confidence intervals (CIs) under five genetic models. The analysis of rs757110 polymorphism showed a significant association with T2DM when comparing T2DM patients with controls (P-values < 0.05). The heterozygous (Bb) and homozygous recessive genotype (bb) showed significant association in T2DM patients with controls showing OR = 1.57 (95%CI [0.96–3.92]), OR = 2.62 (95%CI [1.29–4.89]) respectively. In meta-analysis, significant association was observed between rs757110 SNP and T2DM under allelic OR = 1.23, (95%CI [1.05–1.44]), homozygote OR = 1.58, (95%CI [1.18–2.12]), heterozygote OR = 1.58, (95%CI [1.18–2.12]), dominant OR = 1.26, (95%CI [1.00–1.57]) and recessive OR = 1.29, (95%CI [1.13–1.47]) genetic models in Asian and Caucasian populations. Overall, our findings suggest that rs757110 is positively associated with T2DM susceptibility. However, large-scale studies from various ethnic populations involving multiple risk factors should be performed for better understanding of T2DM pathogenesis.