Genetic association between NLRP3 gene and patients with acne vulgaris

Abstract

Objective The aim was to investigate the genetic association between NACHT, LRR, and PYD Domains-Containing Protein 3 (NLRP3) gene and acne vulgaris among patients. Background Acne vulgaris is a common chronic skin disease. Inflammation is an important pathogenic mechanism of acne, and NLRP3 polymorphisms have been reported to be involved in the mediation and occurrence of the inflammation. However, only a few studies on NLRP3 and acne have been reported, and the mechanism remains unclear. Participants and methods To reach the goal of this research, a case–control study was designed. The study sample included 50 participants divided into two groups: patient group included 40 patients with acne vulgaris having comedones, papules, and nodules, and the control group included 10 healthy age-matched and sex-matched participants. All candidates were subjected to full history taking, clinical examination, BMI, and laboratory investigations for detection of NLRP3 gene single nucleotide polymorphism in DNA extracted from blood samples by real-time polymerase chain reaction. Results We found that taking CC genotype and C allele as references, NLRP3 CG, GG, and CG + GG genotypes and G allele showed significantly higher frequency in all cases compared with control groups (P = 0.030, 0.003, 0.004, and 0.001, respectively). Regarding severity, it was increased significantly in patients carrying CC, CG, and GG, respectively. Most of the severe cases had GG genotype (66.7%), whereas GG genotype was seen in moderate cases (17.6%). GG genotype was significantly associated with severe grades. Conclusion Our study suggests that the NLRP3 SNP rs10754558 is associated with the incidence of (atrioventricular) AV. The G allele might be a genetic risk factor for AV.