Abstract
A NAD-requiring galactose dehydrogenase was discovered in cell-free extracts of two galactose-growing mutants of Ophiostoma multiannulatum. In one of these mutants the enzyme is constitutive, in the other one it is induced by D-galactose. In this last mutant galactose also induces D-xylitol dehydrogenase and D-xylulokinase, which indicates a direct connection between galactose and the pentose phosphate cycle. For induction by galactose of the galactose-1 -phosphate uridyl transferase of the Leloir pathway a functioning galactose dehydrogenase gene is necessary. The mechanism of the co-regulation of the transferase and dehydrogenase genes is not known.
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