Abstract
This is the first study to detection the genetic relationship between Porphyrio alleni and four Rallidae species: Fulica atra , Gallinulla angulata , Gallinulla chloropus and Porphyrio porphyrio . Mitochondrial cytochrome oxidase subunit I (COI) sequences were used as an effective marker in this study. DNA of Rallidae species were extracted, amplified using polymerase chain reaction (PCR) and then sequenced. The results obtained from information based on COI sequences revealed that Gallinulla angulata and Gallinulla chloropus fall into two separate clades and they are not monophyletic. This suggests that, two moorhens could not be laid into the same genus. In addition, Porphyrio porphyrio was included in the same genus with Porphyrio alleni but they were situated in two different clades. Porphyrio alleni was more related to Gallinulla angulate , Gallinulla chloropus and Fulica atra than Porphyrio porphyrio. It was concluded that, the mitochondrial gene COI can aid in the differentiation of studied species and finding genetic relationships between them.
Highlights
Rallidae was greater in number of species other than Gruiformes families
Sequences of five species of Rallidae were compared with records placed in Gen Bank, the results with a cytochrome oxidase subunit I (COI) sequence similar with a record submitted to European Nucleotide Archive (ENA); for Fulica atra; Gallinulla angulata; Gallinulla
The results show that in the topological structures of the tree there are two separate genetic branches, one branch for Porphyrio porphyrio and other branches for Fulica atra; Gallinulla angulata; Gallinulla chloropus; Porphyrio alleni with strong bootstrap support (99%)
Summary
Rallidae was greater in number of species other than Gruiformes families. This diverse Rallidae includes between 135 and 148 known species, comprising nearly 1.3% of recognized birds and 85% of order Gruiform variety, within 33 – 40 genera (Taylor, 1998; Houde, 2009; Clements et al, 2012). These are: Fulica atra (Coot); Gallinulla angulata (Lesser moorhen); Gallinulla chloropus (moorhen); Porphyrio porphyrio (purple swamphen); Porphyrio alleni (Allen's gallinule). These species are belonging to three different genera of family Rallidae termed as, Fulica, Gallinulla and Porphyrio.
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