Abstract
Hybrid sterility is a major hindrance to utilizing the heterosis inindica-japonica hybrids. To isolate a geneSc conferring the hybrid sterility, the locus was mapped using molecular markers and an F2 population derived from a cross between near isogenic lines. A primary linkage analysis showed thatSc was linked closely with 4 markers on chromosome 3, on which the genetic distance between a marker RG227 andSc was 0.07 cM. Chromosome walking with a rice TAC genomic library was carried out using RG227 as a starting probe, and a contig of ca. 320 kb covering theSc locus was constructed. Two TAC clones, M45E14 and M90J01 that might cover theSc locus, were partially sequenced. By searching the rice sequence databases with sequences of the TACs and RG227 ajaponica rice BAC sequence, OSJNBb0078P24 was identified. By comparing the TAC and BAC sequences, six new PCR-based markers were developed. With these markers theSc locus was further mapped to a region of 46 kb. The results suggest that the BAC OSJNBb0078P24 and TAC M45E14 contain theSc gene. Six ORFs were predicted in the focused 46-kb region.
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