Abstract
Transgenic plants of Tylophora indica obtained via somatic embryogenesis through genetic transformation by Agrobacterium rhizogenes have been maintained in vitro for over 6 years and the comparison of long term cultures and field grown normal and transformed plants on various morphological, biochemical and molecular characterisation described. Ri-transformed plants were found to be morphologically stable after 6 years in axenic culture as well as after transfer to the field. Genetic stability for rolA, rolB, rolC and rolD genes were obtained in Ri-transformed plants prior to and after transfer to field. The chromosome number, 2n = 22 was maintained in all the transgenic plants. The Ri-transformed plants retained and expressed all the four rol genes after transfer to the field and were able to synthesise and accumulate tylophorine. Thus, the Ri-transformed plants were found to be stable for more than 6 years in vitro and after 1year of field transfer.
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