Abstract
A breeding program was initiated at the Research Institute of Vegetable Crops, Skierniewice, Poland to select newtomato lines resistant to bacterial speck (Psedomonas syringae pv. tomato (PST) and suitable for field growing. Twotomato cultivars with good agronomic characteristics Luban and Rumba were crossed with resistant to PST ‘Ontario7710’. Then backcross and pedigree breeding procedures were employed to obtain BC5, BC4S1, BC3S2, BC2S3, BC1S4populations. Screening test among those hybrid populations was performed in a greenhouse and exhibited a largeamount of variation in response to PST infection. All plants within BC1S4 progeny of the ‘Luban’ x ‘Ontario 7710’cross and two BC1S4, BC3S2 progenies of the ‘Rumba’ x ‘Ontario 7710’ cross had no symptoms of bacterial speck andcould be assigned to the homozygous category. The remaining progenies were segregating for resistance andsusceptibility and gave distribution of plants over all severity classes. That confirms heterozygosity for resistant genePto of their latter resistant single plant selections before backcrossing or/and selfing. The BC5 populations hadsignificantly higher disease severity rates (DSI = 2.4) than all other selfed populations (DSI = 1.2 – 1.6). After selectionresistant genotypes of the populations were transplanted to the field for morphological traits evaluation. A noticeableprogress in breeding regarding most of the plant and fruit characteristics of the recurrent parents was approached in allpopulations of the ‘Luban’ x ‘Ontario 7710’ cross. Although all progenies of the ‘Rumba’ x ‘Ontario 7710’ differedfrom their recurrent parent as to one feature at least, however the differences showed improved shifting toward thecharacteristics of susceptible parent.
Highlights
MethodsTwo tomato cultivars Luban and Rumba with good agronomic characteristics but susceptible to bacterial speck (PST), and ‘Ontario 7710’ resistant to PST and ten BC5, BC4S1, BC3S2, BC2S3, BC1S4 populations were used in both greenhouse and field experiments
The screening test indicated that severity of bacterial speck on all plants of the recurrent parents ‘Luban’ and ‘Rumba’ was high
Data in this study revealed that plants that carry resistant gene Pto to bacterial speck can be found in all of the tested populations, but genetic backgrounds of the families were different and depended on the homo/heterozygous status of resistant gene Pto
Summary
Two tomato cultivars Luban and Rumba with good agronomic characteristics but susceptible to bacterial speck (PST), and ‘Ontario 7710’ resistant to PST and ten BC5, BC4S1, BC3S2, BC2S3, BC1S4 populations were used in both greenhouse and field experiments. Crosses were made between ‘Rumba’ and ‘Luban’ and the bacterial speck resistant ‘Ontario 7710’. Resistant F1 plants from each of these crosses were used in procedures of backcross and inbreeding based on a one-way program up to the Bc5. After each backcross plants were selfed in consecutive generations. Each population was screened for seedling PST-resistance and only resistant seedlings were transplanted into the field for horticultural traits evaluation (such as plant vigor, fruit size, fruit weight, number of locules) or were transplanted into 10-liter plastic pots under greenhouse conditions for backcrossing or selfing.
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