Abstract
The sublethal effects of water-soluble fraction of gasoline (WSFG, 1.5% v/v) were evaluated in the freshwater fish, Astynax altiparanae, after acute exposure (96h) under a semi-static system. In addition, the recovery process was assessed in the fish following contaminant depuration. Recovery treatments were carried out with gradual depuration (GD), consisting of 7days in the WSFG, followed by 8days in clean water; and treatments with total depuration in clean water for 15 (DEP 15) and 30days (DEP 30). The effects were evaluated through the piscine micronucleus test and by differential counting of organic defense cells. Acute exposure increased the frequency of neutrophils. In the GD treatment, the thrombocyte count and erythrocytic nuclear abnormalities (ENA) increased. In the DEP 15 treatment, there was a reduction of ENA; and following 30days of depuration (i.e., DEP 30), the number of lymphocytes increased and the thrombocyte count remained high. These results indicate a long-term response to a condition of stress from WSFG.
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