Genetic and epigenetic studies on populations of Deschampsia antarctica Desv. from contrasting environments on King George Island

Abstract

Genetic and epigenetic studies on populations ofDeschampsia antarcticaDesv. from contrasting environments on King George IslandPopulations of Antarctic hairgrassDeschampsia antarcticaDesv. from King George Island exhibit variation in many traits. The reason for that is not evident and could be addressed to variable environmental conditions. Obviously, phenotypic variation could be due to stable or temporal changes in expression pattern as the result of adaptation. Stable changes could be due to mutations or site DNA methylation variation that modified expression pattern. Recently, MetAFLP approach was proposed to study such effects. A variant of methylation sensitive AFLP (Amplified Fragment Length Polymorphism), based on the isoschizomeric combinationsAcc65I/MseI andKpnI/MseI was applied to analyze the sequence and site DNA methylation differences between twoD. antarcticapopulations exhibiting morphological dissimilarities. Both DNA sequence mutations and site methylation pattern alternations were detected among and within analyzed populations. It is assumed that such changes might have originated as the response to environmental conditions that induced site methylation alternations leading to phenotypic variation ofD. antarcticapopulations from South Shetland Islands.