Abstract
A new mutant, act, a recessive gene carried on chromosome III of Drosophila, results in sensitivity to actidione, an inhibitor of protein synthesis. The mutant gene has no detectable effect except that it acts as a conditional lethal in the presence of actidione. The possibility that this gene encodes a ribosomal component was tested and eliminated using an in vitro system. Conditions are described for in vitro protein synthesis utilizing Drosophila microsomes and transfer RNA derived from Escherichia coli.
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