Abstract
In mitochondrial DNA, variations in the sequence of 16S rRNA region were analyzed to infer the genetic relationship and population history of three sympatric hipposiderid bats, Hipposideros speoris, H. fulvus and H. ater. Based on the DNA sequence data, we observed relatively lower haplotype and higher nucleotide diversity in H. speoris than in the other two species. The pairwise comparisons of the genetic divergence inferred a genetic relationship between the three hipposiderid bats. We used haplotype sequences to construct a phylogenetic tree. Maximum parsimony and Bayesian inference analysis generated a tree with similar topology. H. fulvus and H. ater formed one cluster and H. speoris formed another cluster. Analysis of the demographic history of populations using Jajima’s D test revealed past changes in populations. Comparison of the observed distribution of pairwise differences in the nucleotides with expected sudden expansion model accepts for H. fulvus and H. ater but not for H. speoris populations.
Highlights
Recent advances in molecular methods have added new insights into studies related to organismic evolution and have revealed unexpected levels of diversity in many vertebrate groups (Meyer et al, 1990; Roca et al, 2001)
The partial sequences of 16S rRNA gene of H. speoris, H. fulvus and H. ater were aligned, and 484 bp data set was considered for phylogenetic analysis
We found 24 distinct haplotypes and their sequences, deposited in GenBank, are available with the following accession number; H. speoris (FJ747652–656, FJ825630–631), H. fulvus (FJ 747658–666), H. ater (FJ747667–674) and R. beddomei (FJ009217)
Summary
Recent advances in molecular methods have added new insights into studies related to organismic evolution and have revealed unexpected levels of diversity in many vertebrate groups (Meyer et al, 1990; Roca et al, 2001) In many such studies, current patterns of genetic variation are used to infer historical events such as population expansions, past population selection from refugia and evolutionary relationship (Miller & Waits, 2003; Johnson & Dunn, 2006; Hoffmann et al, 2008). Postcranial morphology and other morphometric characters, the family Hipposideridae is systematically classified with 65 species, which mainly inhabit tropical and subtropical regions (Hill et al, 1986; Flannery & Colgan, 1993; Wang et al, 2003). Species boundaries within Hipposideridae have been revised several times based on the cranial, morphological and acoustic characteristics, mtDNA and nuclear DNA data (Kingston et al, 2001; Thabah et al, 2006)
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