Abstract
This chapter discusses the genetic analysis of yeast spindle pole bodies. The chapter explains the concepts and methods for three powerful genetic screens that have been fruitful in yielding spindle pole body (SPB) components: a dosage-dependent suppressor screen, an extragenic suppressor screen, and a synthetic lethal screen. An advantage of genetic screens is their sensitivity and encompassing nature. Genetic screens can identify components found in low abundance. Moreover, genetic screens may provide a connection between components that do not physically interact with each other but instead function in the same or parallel pathway. Dosage-dependent suppressor screen searches for genes that suppress a mutant phenotype when present in extra copies. The mechanism of suppression may be direct by promoting binding of one protein to another. In an extragenic suppressor screen, one starts with a given mutant phenotype and searches for genes that are able to suppress this phenotype when mutated. This type of screen can reveal a physical interaction among the products of two genes. Moreover, the basis for synthetic lethal screen concerns the interaction of mutations in two separate genes.
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