Abstract
Stripe rust, caused by the fungal pathogen Puccinia striiformis Westend. f. sp. tritici Eriks, is an important disease of bread wheat (Triticum aestivum L.) worldwide and there is an indication that it may also become a serious disease of durum wheat (T. turgidum L. var. durum). Therefore, we investigated the genetic architecture underlying resistance to stripe rust in adapted durum wheat germplasm. Wheat infection assays were conducted under controlled conditions in Canada and under field conditions in Mexico. Disease assessments were performed on a population of 155 doubled haploid (DH) lines derived from the cross of Kofa (susceptible) and W9262-260D3 (moderately resistant) and on a breeding panel that consisted of 92 diverse cultivars and breeding lines. Both populations were genotyped using the 90K single-nucleotide polymorphism (SNP) iSelect assay. In the DH population, QTL for stripe rust resistance were identified on chromosome 7B (LOD 6.87–11.47) and chromosome 5B (LOD 3.88–9.17). The QTL for stripe rust resistance on chromosome 7B was supported in the breeding panel. Both QTL were anchored to the genome sequence of wild emmer wheat, which identified gene candidates involved in disease resistance. Exome capture sequencing identified variation in the candidate genes between Kofa and W9262-260D3. These genetic insights will be useful in durum breeding to enhance resistance to stripe rust.
Highlights
Durum wheat (Triticum turgidum L. var. durum) is an important food crop in regions with relatively dry climates
When inoculated with W009, 18.7% of the doubled haploid (DH) population had a higher level of resistance than W9262-260D3, while 23.2% were more susceptible than Kofa; ; for W015, 12.3% of the DH population were more resistant than W9262-260D3, while 35.5% were more susceptible than Kofa
The physical locations of the QTL were determined in the wild emmer wheat (WEW) genome and candidate resistance genes and genetic variations within the interval were identified
Summary
Durum wheat (Triticum turgidum L. var. durum) is an important food crop in regions with relatively dry climates.
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