Abstract
Studying nuclear positioning in developing tissues of the model nematode Caenorhabditis elegans greatly contributed to the discovery of SUN and KASH proteins and the formation of the LINC model. Such studies continue to make important contributions into both how LINC complexes are regulated and how defects in LINC components disrupt normal development. The methods described explain how to observe and quantify the following: nuclear migration in embryonic dorsal hypodermal cells, nuclear migration through constricted spaces in larval P cells, nuclear positioning in the embryonic intestinal primordia, and nuclear anchorage in syncytial hypodermal cells. These methods will allow others to employ nuclear positioning in C. elegans as a model to further explore LINC complex regulation and function.
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