Genetic analysis of lncRNA H19 (rs217727) and MIAT (rs1894720) polymorphisms in patients with salivary gland tumors

Abstract

Long noncoding RNAs (lncRNAs) represent a novel pharmacological application in cancer. Salivary gland tumors (SGTs) are essential to both maxillofacial and oral pathology. This study evaluated the H19 (rs217727) and Myocardial infarction-associated transcript (MIAT) (rs1894720) polymorphisms' connection to SGT susceptibility.We studied 104 Iranian patients with clinically definite SGTs and 114 healthy individuals. Samples collected from the participants were prepared, followed by DNA extraction. Following a tetra-primer ARMS-PCR technique employing specific primer pairs, DNA genotyping was carried out.The multivariate logistic regression analysis after adjusting the age revealed that the risk of SGTs was significantly reduced to a 0.17-fold in patients with TT genotype of rs217727 in the co-dominant model relative to CC genotype, while the risk of SGTs increased to 69-fold in patients with GT genotype of rs1894720 in the co-dominant model relative to GG genotype. In the dominant model, the risk of SGTs significantly were increased to a 41.8-fold in patients with GT + TT genotypes of rs1894720 relative to GG genotype. Also, in the recessive model, we found significant decrease in the risk of SGTs in patients with TT genotype of rs217727 compared to CT + CC and also in patients with TT genotype of rs1894720 relative to GT + GG genotype. In over-dominant model, the GT genotypes of rs1894720 significantly were associated to a 69-fold increase the risk of SGTs.Finally, the current study is the first to show that rs217727 and rs1894720 polymorphisms could be related to SGT risk in the Iranian population.