Abstract
Fourteen lipase low-producing (Lip−) mutants of Yarrowia lipolytica were obtained by UV mutagenesis. Complementation studies showed that ten of these mutants belonged to one complementation group. Two other mutants, Lip− 132 and 223, complemented those of the first group. Lip− 1123 complemented mutants of the first and second groups. Lip− 1351 did not complement Lip− 742, 417, 529 and 1133, which belonged to the first group. Genetic recombination analysis of the Lip− mutants by random ascospore analysis showed that mutants of the first and second groups have lesions in the same gene, which is proposed to be the lipase structural gene. Inter-allelic complementation occurs between certain mutants of these groups. Lip− 1123 has a lesion in a different gene.
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