Abstract
Genetic Analysis of Infectious Bronchitis Virus Isolated from Egyptian Broiler Chicken Flocks between 2017 and 2018
Highlights
Infectious Bronchitis (IB) is a highly contagious respiratory viral disease affecting chickens and causes major economic losses to the poultry industry
Detection of Infectious bronchitis virus (IBV) in field samples using qRTPCR Out of 27 tested flocks, nine flocks were positive for IBV using RT-qPCR which were geographically distributed as follow; five positiveflocks out of 14 flocks from Sharkia governorate, one out of four flocks from Dakahliya and oneflockfrom the following governorates (Qalyubiya, Kafrelsheikh and Giza)
Isolation of IBVon embryonated chicken eggs (ECEs) and detection of S1 gene of IBV using conventional RT-PCR Nine isolates, which were positive by RT-qPCR, were subjected for virus isolation and clear pathognomic lesions; curling, stunting, and dwarfing were observed after four blind passages (Figure 1)
Summary
Infectious Bronchitis (IB) is a highly contagious respiratory viral disease affecting chickens and causes major economic losses to the poultry industry. It is characterized by severe respiratory distress in broilers while in layers it causes reduction in egg production (Ahmed and Ruja, 2006; Butcher et al, 2011). S protein is a major structure proteinof IBVwhich is sliced to 2 small protein subunits (S1 and S2) and it is the most variable gene in the IBV genome. S1 gene is about 1644 nucleotide in length and contains three hyper-variable regions (HVR 1, 2, and 3) that are responsible for induction of neutralizing and serotype specific antibodies (Haqshenas et al, 2005).
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