Abstract
The exploitation of heterosis through hybrid breeding is one of the major breeding objectives for productivity increase in crop plants. This research analyzes the genetic basis of heterosis in Brassica juncea by using a doubled haploid (DH) mapping population derived from F1 between two heterotic inbred parents, one belonging to the Indian and the other belonging to the east European gene pool, and their two corresponding sets of backcross hybrids. An Illumina Infinium Brassica 90K SNP array-based genetic map was used to identify yield influencing quantitative trait loci (QTL) related to plant architecture, flowering, and silique- and seed-related traits using five different data sets from multiple trials, allowing the estimation of additive and dominance effects, as well as digenic epistatic interactions. In total, 695 additive QTL were detected for the 14 traits in the three trials using five data sets, with overdominance observed to be the predominant type of effect in determining the expression of heterotic QTL. The results indicated that the design in the present study was efficient for identifying common QTL across multiple trials and populations, which constitute a valuable resource for marker-assisted selection and further research. In addition, a total of 637 epistatic loci were identified, and it was concluded that epistasis among loci without detectable main effects plays an important role in controlling heterosis in yield of B. juncea.
Highlights
Heterosis is defined as the superior performance of F1 hybrids relative to the homozygous parents (East, 1908; Shull, 1908)
The analysis revealed a total of 67 significant genes (p
This study provides the first report on the use of different segregating populations from the same parental combination to study the genetics of heterosis for yield in B. juncea
Summary
Heterosis is defined as the superior performance of F1 hybrids relative to the homozygous parents (East, 1908; Shull, 1908). The development of molecular quantitative genetics has facilitated the study of the genetic basis of heterosis in crops (Paterson et al, 1988; Stuber, 1992). Overdominance has been seen as the primary genetic basis of heterosis in different crops like maize (Stuber et al, 1992), rice (Li et al, 2001; Luo et al, 2001; Mei et al, 2005; Zhu et al, 2016), tomato (Semel et al, 2006; Krieger et al, 2010), and cotton (Ma et al, 2019). Epistasis is defined as the interactions between alleles of different loci and has been shown as the major determinant of heterosis in a few studies (Yu et al, 1997; Hua et al, 2002, 2003; Luo et al, 2009; Tang et al, 2010; Zhou et al, 2012)
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