Genetic Analysis of Heading Date of Japonica Rice Cultivars in Southwest China

Abstract

Heading date of 26 native japonica rice cultivars in southwest China was investigated, and their basic vegetative growth (BVG), photoperiod-sensitivity (PS) and temperature-sensitivity (TS) were analyzed under artificial short-day and natural long-day conditions in Nanjing, as well as artificial high-temperature and natural low-temperature conditions in winter in Hainan. The results showed that the PS and TS varied among different cultivars. The BVG of all the japonica cultivars was well situated, but differed within cultivars. Regression analysis showed a significant correlation between heading date and PS, indicating that PS was the main factor affecting heading date of japonica cultivars in southwest China. Genetic analysis was conducted on these 26 cultivars using a set of heading date near isogenic lines as test lines. All the japonica cultivars carried the dominant early-heading gene Ef-1 or Ef-1 t, and most of these cultivars carried the dominant photoperiod sensitivity allele E 1 or E 1 t, the PS of which was slightly weaker than E 1 . For the Se-1 locus, these cultivars mainly carried recessive photoperiod insensitivity gene Se-1 e. In addition, the PS of 22 japonica cultivars could be repressed or weakened by the recessive allele hd2, inhibiting the expression of E 1 and Se-1. These results indicated that the genotypes of heading date determined different PS and well situated BVG in japonica rice cultivars in southwest China.