Abstract

Back ground: X-chromosomal short tandem repeats (X-STRs) have assured to be informative and particular role in complex relationship testing. DXS6807 known as tetra nucleotides polymorphism representing eight alleles of 251-275 bp in length. DXS6807 is located in, at XP 22.2, at a genetic distance of more than 87 and 151 Cm of X-chromosome. DXS101 is located104.9–121 cM from the Xp-telomere (Xp-tel) corresponding to cytogenetic position in Xq21.33–Xq22.3. 
 Objective: The aim of this present study investigates the allele frequency of two markers DXS101, DXS6807 and forensic efficiency parameters for sample of Arabic Iraqi males.
 Material and methods: The population of this study includes 200 males apparently healthy unrelated participants from different region of Baghdad city, their ages ranged between (20-50) years. The Genomic DNA extracted and purified successfully from blood samples. 
 Results: The forensic efficiency parameters result for these markers were following: polymorphism information content (PIC) of 0.834708, power of discrimination (PD) in male 0.851750, Power of exclusion (PE) 0.698316, MEC Krüger0.511679, MEC Kishida 0.694890. The forensic efficiency parameters analyzing from Arabic population were Power of discrimination (PD) = 0.73405, Polymorphism information content (PIC) =0.69489, Power of exclusion (PE) =0.482879.MEC Krüger =0.511679, MEC Kishida = 0.694890.
 Conclusions: The information provided establish this X-linked microsatellite marker as a valuable strategy for forensic application. DXS101is and DXS6807 recently consider more stable and suitable forensic markers for forensic application.

Highlights

  • The short tandem repeat (STR) markers are typically scattered across the human genome and have been widely researched and applied to forensic analysis and identification of individuals

  • HumDXS6807 is not linked to Hum ARA and Hum HPRTB, which are located at a genetic distance from this region ranged from 80 cM and 150 cM, respectively. these three markers are appropriate for concomitant use in kinship testing and forensic application (5)

  • The allele frequencies and the forensic efficiency parameters of 200 sample calculated for DXS101 separately for males where by 10 alleles specified with length variation that raised in size by (3 bpincrements) ranging from (198–222) bp.As shown in Figure (1): 0.25

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Summary

Introduction

The short tandem repeat (STR) markers are typically scattered across the human genome and have been widely researched and applied to forensic analysis and identification of individuals. Most forensic issue was reporting of autosomal and Y-chromosomal STRs, but it is not widely possible to achieve the population genetic data for X-STR markers.because its usefulness in complex kinship research, X-STRs analysis had earlier attracted the attention of the forensic community. For cases where father / daughter relationships are to be checked, it is advantageous having X-STRs with autosomal markers (1). Because of their specific mode of genetic transmission of hemizygosity in male, X-chromosome STR markers (X-STR) may resolved forensic issues that do not reach the high levels of trust usually needed in legal proceedings, especially with regard to paternities with deficiencies (2).

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