Genetic analysis of clinical varicella-zoster virus isolates collected in China

Abstract

To study the genetic characteristics of vaccine and varicella-zoster virus (VZV) strains isolated from patients with chickenpox or zoster by molecular analysis. SNP based VZV genetic characteristics were analyzed in 19 VZV isolates using the restriction fragment length polymorphisms analysis of DNA fragments of the open reading frames 6, 38, 62 and sequence alignment of the open reading frames 1, 31, 51, 62. All vaccine strains were revealed AluI(-) PstI(-) SmaI(+) BssHII(+) NaeI(+), 96% clinical isolates were revealed AluI(+) PstI(+) SmaI(-) BssHII(-) NaeI(-), 2% clinical isolates were revealed AluI(-) PstI(-) SmaI(+) BssHII(+), 2% clinical isolates were revealed AluI(+) PstI(-) SmaI(-) BssHII(-) NaeI(-) by restriction fragment length polymorphisms analysis and sequence alignment revealed the mutations also presented in this four vaccine strains. Use the restriction fragment length polymorphisms analysis of DNA fragments of the open reading, frames 6 and 62 could be distinguished VZV wild-type strains and vaccine strain in clinical isolates in China. In order to find the adverse effect caused by vaccine from certain company's, analysis on the SNPs in ORFs 1, 31, 51 and 62 is needed.