Abstract
In this study, members of the Carnivore chaphamaparvovirus species 1, closely related to a virus previously reported in dog feces named cachavirus was identified for the first time in feces of Chinese cats. Screening tests using rectal swabs from 171 diarrheic and 378 healthy cats collected from Henan, Anhui, and Zhejiang provinces in China revealed two samples from diarrheic cats that were positive for cachavirus, but statistical analysis indicated no association between the presence of the virus and clinical signs (p > 0.05). Subsequently, two partial genome sequences [from nucleotides 479–4123, according to the strains from dogs (cachavirus)] of the two strains from cats (cachavirus-cat1 and -cat2) were amplified. The NS1 and VP1 sites of cachavirus-cat1 and -cat2 shared a high identity of 91.9 and 97.0% with reported cachaviruses, respectively, but lower identity of 74.8 and 73.2% with another carnivore chaphamaparvovirus named fechaviruses detected in cats, respectively, indicated the two strains might origin from dogs. These findings improve our understanding of the diversity and tropism of viruses in Carnivore chaphamaparvovirus species 1 which now include both dogs and now cats viruses.
Highlights
Parvoviruses belong to the family Parvoviridae, which are small non-enveloped viruses with single-stranded DNA genomes that are ∼4–6 kilobases in length [1]
Studies have reported that expanding its host spectrum of chaphamaparvoviruses, these viruses were identified in mammals such as mice [5, 6], simian [7], swine [8], and Tasmanian devils [9]
Two strains of Cachavirus-1A (MH893826) and Cachavirus-1B (MK448316), were identified in dogs in the United States in 2019, and the cachavirus which belonged to Carnivore chaphamaparvovirus species 1 had three open reading frames (ORFs) encoding a 663-amino acid non-structural protein 1 (NS1), a 504-amino acid capsid protein (VP1) and a 210-amino acid putative nucleoprotein (NP) [14]
Summary
Parvoviruses belong to the family Parvoviridae, which are small non-enveloped viruses with single-stranded DNA (ssDNA) genomes that are ∼4–6 kilobases (kb) in length [1]. Two strains of Cachavirus-1A (MH893826) and Cachavirus-1B (MK448316), were identified in dogs in the United States in 2019, and the cachavirus which belonged to Carnivore chaphamaparvovirus species 1 had three open reading frames (ORFs) encoding a 663-amino acid non-structural protein 1 (NS1), a 504-amino acid capsid protein (VP1) and a 210-amino acid putative nucleoprotein (NP) [14]. Another ChPV named fechavirus was detected in feline outbreak of diarrhea and vomiting and reported in May 2020 [15]. To better understand the host range of members of the Carnivore chaphamaparvovirus species 1, we collected samples from healthy and diarrheic cats to investigate whether the cachavirus or fechavirus was present in cats in China
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