Abstract
The specific-locus test was performed with N-ethyl- N-nitrosourea (ENU) on spermatogonia of mice by using visible loci and a hemoglobin β-chain ( Hbb) locus. Male wild-type (C3Hf/He) mice were injected intraperitoneally with ENU. 8 weeks after injection, these males were mated with tester strain females (PW). 9 visible mutants and the 1 Hbb locus mutant were obtained. This Hbb locus mutation was heritable and viable under homozygous conditions, suggesting that it might have resulted from events at the molecular level.
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