Abstract
1. Improved methods for the culture and preservation ofPhysarum polycephalumamoebae have been developed.2. Strains of amoebae have been isolated which are stably resistant to 4, 8 or 16 μg./ml. actidione.3. Sensitivity vs. resistance to 4 μg./ml. actidione is probably controlled by a single pair of alleles at a locus unlinked to the mating-type locus.4. Plasmodia homozygous or heterozygous for the allele determining resistance at this locus are no more resistant to actidione than plasmodia homozygous for the allele determining sensitivity.
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