Genetic analysis of Acenitobacter bumannii associated with viral respiratory infections

Abstract

In this study, samples were collected from Corona patients who were in isolation units for Corona virus patients, where samples were taken (from the nasal passage, mouth and sputum) and the samples were transferred by a liquid nutrient medium to the laboratory and kept at -2 temperature until they were cultivated on the medium of the Maconkey and saved In the incubator for 24 hours to observe the types of bacteria present , The samples were also cultured on Chrom agar Base Acinetobacter bacteria (which is a selective medium).It is difficult to distinguish it phenotypically from other bacteria, The phenotypic detection of A. bumannii bacteria, biochemical tests, and then molecular detection using ITS-specific primer were also performed and molecular detection using SP4-specific primer were also performed . A molecular assay for the CipA gene that encodes for the production of an enzyme complementary-inhibitor and Plg-binding protein of A. baumannii (Cip A) was also performed and The molecular assay for the Peptidase M10, metallopeptidase-specific primer gene was also performe. As the most important virulence factors in A. bumannii , Because the two enzymes played an important role in the pathogenesis of these bacteria .