Abstract
In the past decade extensive research has been addressed to inducing, selecting, and analyzing specific heritable phenotypic alterations of mammalian cells in culture. The vast literature which has resulted from these efforts has been reviewed on several occasions. The earlier monographs of the nascent discipline could be encyclopedic (De Mars 1971; Thompson and Baker 1973), while some of the more recent treatments ofmammalian cell genetics have been highly specialized and even analytic (e.g. Siminovitch 1976; Basilico 1977; Bakerand Ling1978).Here we will attempt to mark entrance into the “second generation” of somatic cell genetics as characterized by a number of gene transfer techniques and also emphasize very limited specific areas that have not been rigorously discussed previously.
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