Abstract
The increased demand in vegetable oil for food purposes and high-protein feed for livestock and poultry encourages producers to expand the production of various oil crops, while occupying rather cold agroclimatic zones. Improved cold and frost resistance of cultivated crops would significantly increase the yield and expand the range of rape cultivation in a number of cold climate regions. Nine transgenic lines of winter rape containing genes encoding proteins with a cold shock domain (CspA и EsCSDP3) were obtained as a result of Agrobacterium transformation. In total, 260 explants were involved in transformation of rape using pBI121-CSPA-plant, with a transformation efficiency of 2.3%; among 750 explants using the pBI-EsCSDP3 construction, the efficiency was 0.4%. As a result of the studies, it was shown that the expression of the new gene Escsdp3 from the plant of Eutrema salsugineum was able to increase the cold and frost resistance of plants as effectively as the cspa gene from E. coli, which is classically used for this purpose. The cold resistance analysis of T1 transgenic plants generation revealed four cold resistant winter rape lines (three lines with the cspA-plant gene and one line with the Escsdp3 gene). The transfer of Escsdp3 and cspA-plant genes into winter rape plants led to a significant increase in frost resistance of plants. Two winter rapeseed lines were resistant to freezing (with the cspA-plant gene and with the Escsdp3 gene). Non-hardened transgenic plants remained viable after 24 h of exposure to negative temperatures up to −5 °C, and plants that passed through the hardening stage survived after freezing at −16 °C.
Highlights
Most of the total land on Earth has an average minimum temperature
The vector constructs pBI121-CSPA-plant (Figure 1a) and pBI-EsCSDP3 (Figure 1b) were created on the basis of pBI121 vector by replacing the ß-glucoronidase synthesis gene were created on the basis of pBI121 vector by replacing the ß-glucoronidase synthesis which has thehas target from Escherichia coli with the optimized codon gene which the cspA
Finding new genes responsible for abiotic stress tolerance phenotypes is of great importance for a better understanding of stress responses and for promising future crop improvement [17]
Summary
The need for a constant increase in food production while reducing natural resources necessitates the production of plants that can withstand adverse conditions, including the effects of low temperatures. Such abiotic stress as cold stress, including chilling (0–15 ◦ C) and freezing (
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
