Abstract
Carbon dioxide fixation is probably the most important biological reaction to sustain lives of all living maters on this planet, because organic matters indispensable for life are mostly produced from inorganic carbon dioxide by this biological reaction. Hydrogen bacteria are able to grow on carbon dioxide as the sole carbon source by using molecular hydrogen as an energy source. Some kinds of hydrogen bacteria, such as Pseudomonas hydrogenothermophila (Goto et al., 1978) and Hydrogenovibrio marinus (Nishihara et al., 1989, 1991a), have been known to grow extremely fast by using carbon dioxide as the sole carbon source. The specific growth rate for the autotrophic growth of these bacteria are around 0.65-0.70. These bacteria fixes carbon dioxide via the reductive pentose phosphate cycle (Calvin cycle). The key enzyme of the cycle is ribulose-1,5- bisphosphate carboxylase/oxygenase (RubisCO, Ec 4.1.1.39). In this article, we describe our recent results on the structural and related genes for RubisCO from P. hydrogenothermophila and H. marinus.
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