Genes affecting MMP expression are down-regulated in patients with COPD and bronchial asthma

Abstract

Event Abstract Back to Event Genes affecting MMP expression are down-regulated in patients with COPD and bronchial asthma Zdenka Navratilova1*, Tereza Krsjakova2, Eva Novosadova2, Jaromir Zatloukal3, Vitezslav Kolek3 and Martin Petrek1 1 Institute of Molecular and Translational Medicine, Laboratory of Immunogenomics and Immunoproteomics, Czechia 2 Palacky University, Czechia 3 Faculty of Medicine and Dentistry, Palacky University, Dept. of Respiratory Medicine and Tuberculosis, Czechia Proteolytic enzymes such as matrix metalloproteinases (MMPs) and others are up-regulated in chronic obstructive pulmonary disease (COPD) and bronchial asthma (BA). Their exceeding expression and proteolytic activity could result from insufficient inhibition and deregulated mRNA stability. We, therefore, investigated two inhibitors of MMPs (RECK and PTEN) and also RNA-binding proteins affecting mRNA stability (AUF1, HuR and PCBP2) in the both diseases. Real time-PCR method with PSMB2 as housekeeping gene was used to quantify the relative expression of RECK, PTEN, AUF1, HuR and PCBP2 in bronchoalveolar cells from 22 control subjects, 30 COPD patients and 19 patients with BA. The numbers of RECK (p=0.004, p=0.03)and AUF1 (p=0.01, p=0.02) mRNA transcripts were lower in both groups of COPD and BA patients than those in healthy control subjects. In comparison to controls, mRNA expression of HuR was decreased in COPD patients (p=0.03). There was a positive correlation between RECK expression and AUF1 (p<0.001), PCBP2 (p<0.001) and HuR (p=0.02), respectively. Overall, expression of the investigated genes did not differ between COPD patients and those with BA (p>0.05), smoking status was reflected in our analyses. In conclusion, the down-regulation of RECK observed in this study may contribute to protease/anti-protease imbalance in COPD and BA. As this has been the first investigation of RNA-binding proteins in these inflammatory diseases, further studies are needed to clarify the involvement of these molecules in COPD and/or BA pathomechanisms. Grant support IGA PU LF 2013_009 and CZ.1.05./2.1.00/01.0030 Keywords: COPD, Asthma, RNA-Binding Proteins, inhibitors of MMPs, metalloproteinase Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Immune-mediated disease pathogenesis Citation: Navratilova Z, Krsjakova T, Novosadova E, Zatloukal J, Kolek V and Petrek M (2013). Genes affecting MMP expression are down-regulated in patients with COPD and bronchial asthma. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00291 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 14 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Zdenka Navratilova, Institute of Molecular and Translational Medicine, Laboratory of Immunogenomics and Immunoproteomics, Olomouc, Czechia, navratilz@yahoo.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Zdenka Navratilova Tereza Krsjakova Eva Novosadova Jaromir Zatloukal Vitezslav Kolek Martin Petrek Google Zdenka Navratilova Tereza Krsjakova Eva Novosadova Jaromir Zatloukal Vitezslav Kolek Martin Petrek Google Scholar Zdenka Navratilova Tereza Krsjakova Eva Novosadova Jaromir Zatloukal Vitezslav Kolek Martin Petrek PubMed Zdenka Navratilova Tereza Krsjakova Eva Novosadova Jaromir Zatloukal Vitezslav Kolek Martin Petrek Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.