Generation time ofCandida albicansin synchronized and nonsynchronized cultures

Abstract

Synchronized growth of Candida albicans was induced. The generation time of the synchronized cells was compared with those of nonsynchronized cells cultured in Sabouraud broth, Medium 199, and a synthetic synchronization medium. The mean generation time of the synchronized cultures was 3·2 hours while that of the nonsynchronized cultures ranged from 1·7 to 3·6 hours. The generation time in shake cultures of nonsynchronized cells was uniformly shorter than that of the corresponding aerated cultures. The generation time in Sabouraud medium was the shortest and that in synthetic synchronization medium was the longest with each method of incubation.