Abstract
Transcription factors (TFs) play important roles in plant growth, development, and responses to environmental stress. In this study, we collected 1,455 full-length (FL) cDNAs of TFs, representing 45 families, from wheat and its relatives Triticum urartu, Aegilops speltoides, Aegilops tauschii, Triticum carthlicum, and Triticum aestivum. More than 15,000 T0 TF FOX (Full-length cDNA Over-eXpressing) rice lines were generated; of these, 10,496 lines set seeds. About 14.88% of the T0 plants showed obvious phenotypic changes. T1 lines (5,232 lines) were screened for salt and osmotic stress tolerance using 150 mM NaCl and 20% (v/v) PEG-4000, respectively. Among them, five lines (591, 746, 1647, 1812, and J4065) showed enhanced salt stress tolerance, five lines (591, 746, 898, 1078, and 1647) showed enhanced osmotic stress tolerance, and three lines (591, 746, and 1647) showed both salt and osmotic stress tolerance. Further analysis of the T-DNA flanking sequences showed that line 746 over-expressed TaEREB1, line 898 over-expressed TabZIPD, and lines 1812 and J4065 over-expressed TaOBF1a and TaOBF1b, respectively. The enhanced salt and osmotic stress tolerance of lines 898 and 1812 was confirmed by retransformation of the respective genes. Our results demonstrate that a heterologous FOX system may be used as an alternative genetic resource for the systematic functional analysis of the wheat genome.
Highlights
With a global output of 681 million tons in 2011, bread wheat (Triticum aestivum; AABBDD)accounts for 20% of the calories consumed by humans and is an important source of proteins, vitamins, and minerals [1]
Our results demonstrate that a heterologous FOX system may be used as an alternative genetic resource for the systematic functional analysis of the wheat genome
FOX gene hunting enables the systematic dissection of gene function without knowing the complete genome sequence of the organism of interest
Summary
With a global output of 681 million tons in 2011, bread wheat (Triticum aestivum; AABBDD)accounts for 20% of the calories consumed by humans and is an important source of proteins, vitamins, and minerals [1]. With a global output of 681 million tons in 2011, bread wheat (Triticum aestivum; AABBDD). Bread wheat is thought to have originated as a result of hybridization between the wild diploid grass Aegilops tauschii (DD) and the cultivated tetraploid wheat. Triticum dicoccoides (AABB) [1]. The large size and complexity of the wheat genome have been substantial barriers to functional analyses of its genes. Scientists published draft sequences of the AABBDD genome of the hexaploid wheat variety Chinese Spring (CS42) [1], the genome of the wheat A-genome progenitor Triticum urartu accession G1812, and the DD genome of Ae. tauschii accession AL8/78 [2, 3]. The estimated relative genome sizes are about
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