Generation of two human isogenic iPSC lines from fetal dermal fibroblasts

Rashmi Tandon,Björn Brändl,Natalya Baryshnikova,Alexandro Landshammer,Laura Steenpaß,Oliver Keminer,Ole Pless,Franz-Josef Müller

doi:10.1016/j.scr.2018.10.004

Abstract

Two isogenic hiPSC lines, ZIPi013-B and ZIPi013-E, were generated by reprogramming fetal dermal fibroblasts with episomal vectors. Previously, the same fetal fibroblasts were reprogrammed multiple times in a study comparing other reprogramming methods. As a consequence, the genomes have been sequenced multiple times. Both new cell lines offer the opportunity to study basic stem cell biology and model human disease. They can be applied as reference cell lines for creating isogenic clones bearing disease mutations on a well-characterized genomic background, as both cell lines have demonstrated excellent differentiation capacity in multiple labs.Resource tableUnlabelled TableUnique stem cell lines identifierZIPi013-BZIPi013-EAlternative names of stem cell linesZIP13K2 (ZIPi013-B)ZIP13K5 (ZIPi013-E)InstitutionZentrum für Integrative Psychiatrie gGmbH, Kiel, GermanyContact information of distributorPD Dr. Franz-Josef Müller, franz-josef.mueller@uksh.deType of cell linesiPSCOriginhumanCell SourceFibroblastsClonalityClonalMethod of reprogrammingTransgene free, episomalMultiline rationaleIsogenic clonesGene modificationNOType of modificationN/AAssociated diseaseN/AGene/locusN/AMethod of modificationN/AName of transgene or resistanceN/AInducible/constitutive systemN/ADate archived/stock dateStock date ZIPi013-B 8th December 2017, stock date ZIPi013-E 12th December 2017Cell line repository/bankN/AEthical approvalhttps://www.sciencellonline.com/technical-support/ethical-statement.htmlEthikkommission der medizinischen Fakultät der Christian-Albrechts-Universität zu Kiel, approval number A145/11

Highlights

Two isogenic hiPSC lines, ZIPi013-B and ZIPi013-E, were generated by reprogramming fetal dermal fibroblasts with episomal vectors
We generated ZIPi013-B and ZIPi013-E cell lines from fetal dermal fibroblasts, following the reprogramming protocol described by Okita et al, 2011 (Okita et al, 2011)
A quantitative assessment of pluripotency markers by script-based counting of immunostained cells showed more than 90% of cells in both hiPSC lines are positive for SOX2, OCT-3/4, SSEA-4 and TRA–1–60 expression (Fig. 1A, B)

Summary

Introduction

Two isogenic hiPSC lines, ZIPi013-B and ZIPi013-E, were generated by reprogramming fetal dermal fibroblasts with episomal vectors. We generated ZIPi013-B and ZIPi013-E cell lines from fetal dermal fibroblasts, following the reprogramming protocol described by Okita et al, 2011 (Okita et al, 2011). We performed immunocytochemistry for SOX2, NANOG, OCT-3/4, SSEA-4 and TRA–1–60 (Fig. 1A, scale bar 100 μm) to demonstrate the pluripotent-like state of generated hiPSC lines.

Results

Conclusion