Generation of two human control iPS cell lines (UCLi016-A and UCLi017-A) from healthy donors with no known ocular conditions.

Mariya Moosajee,Philippa Harding,Dulce Lima Cunha,Hajrah Sarkar,Cécile Méjécase,Lyes Toualbi,Jonathan Eintracht

doi:10.1016/j.scr.2020.102113

Abstract

Two human induced pluripotent stem cell (hiPSC) lines (UCLi016-A and UCLi017-A) were generated from fibroblast cells of 23- and 34-year-old healthy male donors with no known ocular conditions. Fibroblast cells were derived from skin biopsies and reprogrammed using integration free episomal reprogramming. The established iPSC lines were found to express pluripotency markers, exhibit differentiation potential in vitro and display a normal karyotype. These cell lines will act as a control lines for researchers studying ocular diseases.

Highlights

Two human induced pluripotent stem cell lines (UCLi016-A and UCLi017-A) were generated from fibroblast cells of 23- and 34-year-old healthy male donors with no known ocular conditions
Resource utility Human induced pluripotent stem cell generated from two healthy male donors with no known ocular conditions using integrationfree episomal reprogramming from fibroblasts will act as control lines for studying cellular models of ocular disease
Two iPSC lines were derived from the fibroblasts of the healthy male donors with no known ocular conditions (Table 1)

Summary

Resource table

2. Resource utility Human induced pluripotent stem cell (hiPSCs) generated from two healthy male donors with no known ocular conditions using integrationfree episomal reprogramming from fibroblasts will act as control lines for studying cellular models of ocular disease. Two iPSC lines were derived from the fibroblasts of the healthy male donors with no known ocular conditions (Table 1) These iPSC lines can be used as control lines for research into cellular models of ocular disease. Two wild-type (WT) control hiPSCs were generated from fibroblasts of healthy male donors with no known ocular condi tions These iPSC lines will be used as control lines for disease modelling, to aid in understanding molecular pathology of ocular disease, identi fying therapeutic targets and drug screening

Fibroblast derivation and culture

Fibroblast reprogramming and iPSC culture

Immunocytochemistry

Result

Embryoid body mediated spontaneous in vitro differentiation

Low-pass whole genome sequencing and STR analysis