Generation of transgenic ducks by crispr/CAS9-mediated gene inser-tion combined with the sperm-mediated gene transfer (SMGT)

O Konoval,S Kostenko,L Lizhi,B Xingchen,H Xuetao,M Drahulian,P Tabaka,M Doroshenko,P Korol,L Liumeng,A Chepiha

doi:10.7124/bc.000a16

Abstract

Generation of transgenic ducks by crispr/CAS9-mediated gene inser-tion combined with the sperm-mediated gene transfer (SMGT)

Highlights

The bird has been used for a long time as a model of the embryonic development [1]
Anas platyrhynchos has not been studied thoroughly compared with other poultry, and there are no reported studies on a reliable genome engineering technique in the duck
The 31 F1 ducks were mated with non-transgened ducks to obtain their F2 offsprings

Summary

Introduction

The bird has been used for a long time as a model of the embryonic development [1]. The bird egg is a potential bioreactor for heterologous production of protein, especially for the production of recombinant therapeutic proteins in biopharmaceutical industry [2,3,4,5]. Birds have several advantages compared with other species: the short generation interval, high protein eggs, and good sperm production. The vast majority of studies on the transgenic poultry are focused on chickens (Gallus gallus) [4] and quails (Coturnix japonica) [7]. The eggs of duck (Anas platyrhynchos) are larger than the chicken and quail eggs [8], and have an advantage over the latter due to a possibility of usage as bioreactors. Anas platyrhynchos has not been studied thoroughly compared with other poultry, and there are no reported studies on a reliable genome engineering technique in the duck. Such work is of high significance for further use of ducks as a model of waterfowl species. It is necessary to pay a special attention to the egg-laying duck breeds characterized by a high level of egg productivity, rather than to the meat duck breeds

Methods

Results

Discussion

Conclusion