Abstract
This study illustrates the preparation of in situ generated TiO2 nanoparticle-based bio-composite and its application in pathogen removal. Pristine cellulose acetate gel was mixed with acacia extract impregnated egg shell and coated with TiO2 nanoparticles. The resultant biocomposites were used as coatings and compared for their performances. All prepared coatings were analysed using Fourier transform infrared spectroscopy, scanning electron microscope, Energy-dispersive X-ray spectroscopy, scanning probe microscopy, thermogravimetric analysis and contact angle measurement. The antimicrobial activity of bio-composite was tested on E. coli bacteria using plating and fluorescent based assay. The bio-composites showed very good antimicrobial properties. Moreover, the easy and eco-friendly method for developing such bio-composite coatings and their application may prove effective against pathogens during effluent treatment.
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