Abstract
We previously reported successful establishment of retinal progenitor cell clones. Here, we cultured them on temperaturereversible gelation polymer, with supplementation of several chemokines. The retinal progenitor cell clones formed a cell sheet with extension of their axon like processes. RT-PCR of the cell sheets after two week culture, they were positive for retinal ganglion cell markers, bipolar cell markers, and photoreceptor cell markers. Immunohistochemistry suggested that the cell sheets contained photoreceptors and synaptic connection was observed within the sheet. We found that in vitro culture of cloned retinal progenitor cells led to formation of the cell sheet being rhodopsin positive. The cell sheet made of photoreceptor cells may have the potential to benefit various retinal diseases.
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