Abstract
Background Multipotent stem cells have been isolated from multiple sources including, bone marrow, adipose tissue, placenta, umbilical cord and cardiac tissue. It is predicted that large numbers of therapeutically-active cells isolated from these tissue sources will be required to treat patients inflicted with various disorders. Experimental evidence suggests that these various cell types can exhibit distinct characteristics depending upon tissue source and method of expansion: differential expression of cellular markers is sometimes detected, doubling times and expansion limits can differ, and physical differences that influence the ability of cells to adhere to various synthetic surfaces are observed. A novel prototype microcarrier recently developed by Pall promotes rapid attachment and growth of multiple cell types in stirredtank reactors. Additionally, peptide-coating provides an alternative animal component-free substrate for cell expansion. These desirable attributes manifest in both serum-containing and animal component-free medium formulations.
Highlights
Multipotent stem cells have been isolated from multiple sources including, bone marrow, adipose tissue, placenta, umbilical cord and cardiac tissue
It is predicted that large numbers of therapeutically-active cells isolated from these tissue sources will be required to treat patients inflicted with various disorders
Experimental evidence suggests that these various cell types can exhibit distinct characteristics depending upon tissue source and method of expansion: differential expression of cellular markers is sometimes detected, doubling times and expansion limits can differ, and physical differences that influence the ability of cells to adhere to various synthetic surfaces are observed
Summary
Multipotent stem cells have been isolated from multiple sources including, bone marrow, adipose tissue, placenta, umbilical cord and cardiac tissue. It is predicted that large numbers of therapeutically-active cells isolated from these tissue sources will be required to treat patients inflicted with various disorders. Experimental evidence suggests that these various cell types can exhibit distinct characteristics depending upon tissue source and method of expansion: differential expression of cellular markers is sometimes detected, doubling times and expansion limits can differ, and physical differences that influence the ability of cells to adhere to various synthetic surfaces are observed. A novel prototype microcarrier recently developed by Pall promotes rapid attachment and growth of multiple cell types in stirredtank reactors. Peptide-coating provides an alternative animal component-free substrate for cell expansion. These desirable attributes manifest in both serum-containing and animal component-free medium formulations
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